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Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology.
Bai, Yunhao; Zhu, Bokai; Oliveria, John-Paul; Cannon, Bryan J; Feyaerts, Dorien; Bosse, Marc; Vijayaragavan, Kausalia; Greenwald, Noah F; Phillips, Darci; Schürch, Christian M; Naik, Samuel M; Ganio, Edward A; Gaudilliere, Brice; Rodig, Scott J; Miller, Michael B; Angelo, Michael; Bendall, Sean C; Rovira-Clavé, Xavier; Nolan, Garry P; Jiang, Sizun.
Afiliação
  • Bai Y; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Zhu B; Department of Chemistry, Stanford University, Stanford, CA, USA.
  • Oliveria JP; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Cannon BJ; Department of Microbiology and Immunology, Stanford University, Stanford, CA, USA.
  • Feyaerts D; Department of Translational Medicine, Genentech, Inc., South San Francisco, CA, USA.
  • Bosse M; Department of Medicine, McMaster University, Hamilton, ON, Canada.
  • Vijayaragavan K; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Greenwald NF; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, USA.
  • Phillips D; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Schürch CM; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Naik SM; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Ganio EA; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Gaudilliere B; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Rodig SJ; Department of Pathology and Neuropathology, University Hospital and Comprehensive Cancer Center Tübingen, Tübingen, Germany.
  • Miller MB; Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Angelo M; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, USA.
  • Bendall SC; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, USA.
  • Rovira-Clavé X; Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Nolan GP; Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Jiang S; Department of Neurology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Nat Commun ; 14(1): 4013, 2023 07 07.
Article em En | MEDLINE | ID: mdl-37419873
ABSTRACT
Cellular organization and functions encompass multiple scales in vivo. Emerging high-plex imaging technologies are limited in resolving subcellular biomolecular features. Expansion Microscopy (ExM) and related techniques physically expand samples for enhanced spatial resolution, but are challenging to be combined with high-plex imaging technologies to enable integrative multiscaled tissue biology insights. Here, we introduce Expand and comPRESS hydrOgels (ExPRESSO), an ExM framework that allows high-plex protein staining, physical expansion, and removal of water, while retaining the lateral tissue expansion. We demonstrate ExPRESSO imaging of archival clinical tissue samples on Multiplexed Ion Beam Imaging and Imaging Mass Cytometry platforms, with detection capabilities of > 40 markers. Application of ExPRESSO on archival human lymphoid and brain tissues resolved tissue architecture at the subcellular level, particularly that of the blood-brain barrier. ExPRESSO hence provides a platform for extending the analysis compatibility of hydrogel-expanded biospecimens to mass spectrometry, with minimal modifications to protocols and instrumentation.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas / Microscopia Tipo de estudo: Guideline Limite: Humans Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
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PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas / Microscopia Tipo de estudo: Guideline Limite: Humans Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos