Identification of N-degrons and N-recognins using peptide pull-downs combined with quantitative mass spectrometry.

Müller, Franziska; Bange, Tanja

Müller, Franziska; Bange, Tanja.

Afiliação

Müller F; Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany.
Bange T; Institute of Medical Psychology, Faculty of Medicine, Ludwig-Maximilians University, Munich, Germany. Electronic address: tanja.bange@med.uni-muenchen.de.

Methods Enzymol ; 686: 67-97, 2023.

Article em En | MEDLINE | ID: mdl-37532409

Assuntos

Peptídeos; Ubiquitina-Proteína Ligases; Peptídeos/química; Proteólise; Ubiquitina-Proteína Ligases/metabolismo; Ubiquitina/metabolismo; Espectrometria de Massas

Palavras-chave

E3 ligase; Label-free quantification; Mass-spectrometry; N-degron; N-recognin; N-terminal acetylation; N-terminal modification; Peptide pull-down; Protein quality control and stability; Quantitative MS-based proteomics

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peptídeos / Ubiquitina-Proteína Ligases Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Methods Enzymol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Alemanha

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