Bifidobacterium infantis utilizes N-acetylglucosamine-containing human milk oligosaccharides as a nitrogen source.
Gut Microbes
; 15(2): 2244721, 2023 12.
Article
em En
| MEDLINE
| ID: mdl-37609905
ABSTRACT
Bifidobacterium longum subsp. infantis (B. infantis) utilizes oligosaccharides secreted in human milk as a carbohydrate source. These human milk oligosaccharides (HMOs) integrate the nitrogenous residue N-acetylglucosamine (NAG), although HMO nitrogen utilization has not been described to date. Herein, we characterize the B. infantis nitrogen utilization phenotype on two NAG-containing HMO species, LNT and LNnT. This was characterized through in vitro growth kinetics, incorporation of isotopically labeled NAG nitrogen into the proteome, as well as modulation of intracellular 2-oxoglutarate levels while utilizing HMO nitrogen. Further support is provided by comparative transcriptomics and proteomics that identified global regulatory networks deployed during HMO nitrogen utilization. The aggregate data demonstrate that B. infantis strains utilize HMO nitrogen with the potential to significantly impact fundamental and clinical studies, as well as enable applications.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Microbioma Gastrointestinal
/
Bifidobacterium longum subspecies infantis
Limite:
Humans
Idioma:
En
Revista:
Gut Microbes
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Estados Unidos