Anaplasma phagocytophilum in European bison (Bison bonasus) and their ticks from Lithuania and Poland.
Ticks Tick Borne Dis
; 14(6): 102246, 2023 11.
Article
em En
| MEDLINE
| ID: mdl-37639831
ABSTRACT
The increasing population of European bison (Bison bonasus) can contribute to the prevalence of zoonotic pathogens. The aim of the present study was to assess the presence of A. phagocytophilum infection in European bison tissues as well as ticks removed from European bison in Lithuania and Poland. A further objective of this work was to compare the detected A. phagocytophilum strains. A total of 85 tissue samples (spleen) of European bison and 560 ticks belonging to two species, Ixodes ricinus (n = 408) and Dermacentor reticulatus (n = 152) were tested. DNA of A. phagocytophilum was detected based on RT-PCR in 40% of the European bison samples, 8.8% of the I. ricinus and 5.9% of the D. reticulatus ticks. Analysis of the obtained partial 16S rRNA gene sequences of A. phagocytophilum revealed the presence of three variants with two polymorphic sites. Furthermore, phylogenetic analysis with partial msp4 gene sequences grouped A. phagocytophilum variants into three clusters. This study revealed that the groEL gene sequences of A. phagocytophilum from European bison and their ticks grouped into ecotype I and only one sequence from Lithuanian European bison belonged to ecotype II. The results of the present study indicated that European bison may play a role as a natural reservoir of A. phagocytophilum.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bison
/
Ixodes
/
Anaplasma phagocytophilum
/
Dermacentor
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
País/Região como assunto:
Europa
Idioma:
En
Revista:
Ticks Tick Borne Dis
Ano de publicação:
2023
Tipo de documento:
Article