Superior Photoprotection of Cyanine Dyes with Thio-imidazole Amino Acids.
J Am Chem Soc
; 145(36): 19571-19577, 2023 09 13.
Article
em En
| MEDLINE
| ID: mdl-37658476
ABSTRACT
Preventing fluorophore photobleaching and unwanted blinking is crucial for single-molecule fluorescence (SMF) studies. Reductants achieve photoprotection via quenching excited triplet states, yet either require counteragents or, for popular alkyl-thiols, are limited to cyanine dye Cy3 protection. Here, we provide mechanistic and imaging results showing that the naturally occurring amino acid ergothioneine and its analogue dramatically enhance photostability for Cy3, Cy5, and their conformationally restrained congeners, providing a biocompatible universal solution for demanding fluorescence imaging.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Quinolinas
/
Ergotioneína
Idioma:
En
Revista:
J Am Chem Soc
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Canadá