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Comprehensive autoantibody profiling in systemic autoimmunity by a highly-sensitive multiplex protein array.
Kuzumi, Ai; Norimatsu, Yuta; Matsuda, Kazuki M; Ono, Chihiro; Okumura, Taishi; Kogo, Emi; Goshima, Naoki; Fukasawa, Takemichi; Fushida, Natsumi; Horii, Motoki; Yamashita, Takashi; Yoshizaki-Ogawa, Asako; Yamaguchi, Kei; Matsushita, Takashi; Sato, Shinichi; Yoshizaki, Ayumi.
Afiliação
  • Kuzumi A; Department of Dermatology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
  • Norimatsu Y; Department of Dermatology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
  • Matsuda KM; Department of Dermatology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
  • Ono C; ProteoBridge Corporation, Tokyo, Japan.
  • Okumura T; ProteoBridge Corporation, Tokyo, Japan.
  • Kogo E; ProteoBridge Corporation, Tokyo, Japan.
  • Goshima N; ProteoBridge Corporation, Tokyo, Japan.
  • Fukasawa T; Molecular Profiling Research Center for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tokyo, Japan.
  • Fushida N; Department of Dermatology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
  • Horii M; Department of Clinical Cannabinoid Research, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
  • Yamashita T; Department of Dermatology, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
  • Yoshizaki-Ogawa A; Department of Dermatology, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
  • Yamaguchi K; Department of Dermatology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
  • Matsushita T; Department of Dermatology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
  • Sato S; ProteoBridge Corporation, Tokyo, Japan.
  • Yoshizaki A; Molecular Profiling Research Center for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tokyo, Japan.
Front Immunol ; 14: 1255540, 2023.
Article em En | MEDLINE | ID: mdl-37701440
ABSTRACT
Comprehensive autoantibody evaluation is essential for the management of autoimmune disorders. However, conventional methods suffer from poor sensitivity, low throughput, or limited availability. Here, using a proteome-wide human cDNA library, we developed a novel multiplex protein assay (autoantibody array assay; A-Cube) covering 65 antigens of 43 autoantibodies that are associated with systemic sclerosis (SSc) and polymyositis/dermatomyositis (PM/DM). The performance of A-Cube was validated against immunoprecipitation and established enzyme-linked immunosorbent assay. Further, through an evaluation of serum samples from 357 SSc and 172 PM/DM patients, A-Cube meticulously illustrated a diverse autoantibody landscape in these diseases. The wide coverage and high sensitivity of A-Cube also allowed the overlap and correlation analysis between multiple autoantibodies. Lastly, reviewing the cases with distinct autoantibody profiles by A-Cube underscored the importance of thorough autoantibody detection. Together, these data highlighted the utility of A-Cube as well as the clinical relevance of autoantibody profiles in SSc and PM/DM.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Escleroderma Sistêmico / Doenças Autoimunes / Dermatomiosite Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Front Immunol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Japão
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Escleroderma Sistêmico / Doenças Autoimunes / Dermatomiosite Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Front Immunol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Japão