Genetic signatures associated with the virulence of porcine epidemic diarrhea virus AH2012/12.
J Virol
; 97(10): e0106323, 2023 10 31.
Article
em En
| MEDLINE
| ID: mdl-37732788
ABSTRACT
IMPORTANCE Porcine epidemic diarrhea (PED) caused by PED virus (PEDV) remains a big threat to the swine industry worldwide. Vaccination with live attenuated vaccine is a promising method to prevent and control PED, because it can elicit a more protective immunity than the killed vaccine, subunit vaccine, and so on. In this study, we found two obvious deletions in the genome of a high passage of AH2012/12. We further confirmed the second deletion which contains seven amino acids at the carboxy-terminus of the S2 gene and the start codon of ORF3 can reduce its pathogenicity in vivo. Animal experiments indicated that the recombinant PEDV with deleted carboxy-terminus of S gene showed higher IgG, IgA, neutralization antibodies, and protection effects against virus challenge than the killed vaccine. These data reveal that the engineering of the carboxy-terminus of the S2 gene may be a promising method to develop live attenuated vaccine candidates of PEDV.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doenças dos Suínos
/
Infecções por Coronavirus
/
Vírus da Diarreia Epidêmica Suína
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
J Virol
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Namíbia