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The localization of centromere protein A is conserved among tissues.
Cappelletti, Eleonora; Piras, Francesca M; Sola, Lorenzo; Santagostino, Marco; Petersen, Jessica L; Bellone, Rebecca R; Finno, Carrie J; Peng, Sichong; Kalbfleisch, Ted S; Bailey, Ernest; Nergadze, Solomon G; Giulotto, Elena.
Afiliação
  • Cappelletti E; Department of Biology and Biotechnology, University of Pavia, Pavia, Italy.
  • Piras FM; Department of Biology and Biotechnology, University of Pavia, Pavia, Italy.
  • Sola L; Department of Biology and Biotechnology, University of Pavia, Pavia, Italy.
  • Santagostino M; Department of Biology and Biotechnology, University of Pavia, Pavia, Italy.
  • Petersen JL; Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE, USA.
  • Bellone RR; Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis, Davis, CA, USA.
  • Finno CJ; Veterinary Genetics Laboratory, School of Veterinary Medicine, University of California-Davis, Davis, CA, USA.
  • Peng S; Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis, Davis, CA, USA.
  • Kalbfleisch TS; Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis, Davis, CA, USA.
  • Bailey E; Gluck Equine Research Center, University of Kentucky, Lexington, KY, USA.
  • Nergadze SG; Gluck Equine Research Center, University of Kentucky, Lexington, KY, USA.
  • Giulotto E; Department of Biology and Biotechnology, University of Pavia, Pavia, Italy.
Commun Biol ; 6(1): 963, 2023 09 21.
Article em En | MEDLINE | ID: mdl-37735603
Centromeres are epigenetically specified by the histone H3 variant CENP-A. Although mammalian centromeres are typically associated with satellite DNA, we previously demonstrated that the centromere of horse chromosome 11 (ECA11) is completely devoid of satellite DNA. We also showed that the localization of its CENP-A binding domain is not fixed but slides within an about 500 kb region in different individuals, giving rise to positional alleles. These epialleles are inherited as Mendelian traits but their position can move in one generation. It is still unknown whether centromere sliding occurs during meiosis or during development. Here, we first improve the sequence of the ECA11 centromeric region in the EquCab3.0 assembly. Then, to test whether centromere sliding may occur during development, we map the CENP-A binding domains of ECA11 using ChIP-seq in five tissues of different embryonic origin from the four horses of the equine FAANG (Functional Annotation of ANimal Genomes) consortium. Our results demonstrate that the centromere is localized in the same region in all tissues, suggesting that the position of the centromeric domain is maintained during development.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA Satélite / Centrômero Limite: Animals / Humans Idioma: En Revista: Commun Biol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Itália

Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA Satélite / Centrômero Limite: Animals / Humans Idioma: En Revista: Commun Biol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Itália