Harvesting mouse suprachiasmatic nucleus by vibrating microtome for diurnal transcriptome analysis.
STAR Protoc
; 4(4): 102618, 2023 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-37756154
ABSTRACT
The mammalian suprachiasmatic nucleus (SCN) is the principal circadian clock that synchronizes daily behavioral and physiological responses in response to environmental cues. Here, we present a protocol for harvesting mouse SCN by vibrating microtome for diurnal transcriptome analysis. We describe steps for mouse entrainment, isolation of the SCN, tissue preparation, slicing with a vibratome, and handling of the harvested SCN for RNA extraction. This protocol can also be used for harvesting other mammalian brain regions for genomic studies.
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Base de dados:
MEDLINE
Assunto principal:
Ritmo Circadiano
/
Relógios Circadianos
Limite:
Animals
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2023
Tipo de documento:
Article