Gene recoding by synonymous mutations creates promiscuous intragenic transcription initiation in mycobacteria.
mBio
; 14(5): e0084123, 2023 Oct 31.
Article
em En
| MEDLINE
| ID: mdl-37787543
ABSTRACT
IMPORTANCE Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis, one of the deadliest infectious diseases worldwide. Previous studies have established that synonymous recoding to introduce rare codon pairings can attenuate viral pathogens. We hypothesized that non-optimal codon pairing could be an effective strategy for attenuating gene expression to create a live vaccine for Mtb. We instead discovered that these synonymous changes enabled the transcription of functional mRNA that initiated in the middle of the open reading frame and from which many smaller protein products were expressed. To our knowledge, this is one of the first reports that synonymous recoding of a gene in any organism can create or induce intragenic transcription start sites.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Mutação Silenciosa
/
Mycobacterium
Idioma:
En
Revista:
MBio
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Estados Unidos