Characterization of low-grade epilepsy-associated tumor from implanted stereoelectroencephalography electrodes.
Epilepsia Open
; 9(1): 409-416, 2024 Feb.
Article
em En
| MEDLINE
| ID: mdl-37798921
ABSTRACT
Low-grade epilepsy-associated tumors (LEATs) are a common cause of drug-resistant epilepsy in children. Herein, we demonstrate the feasibility of using tumor tissue derived from stereoelectroencephalography (sEEG) electrodes upon removal to molecularly characterize tumors and aid in diagnosis. An 18-year-old male with focal epilepsy and MRI suggestive of a dysembryoplastic neuroepithelial tumor (DNET) in the left posterior temporal lobe underwent implantation of seven peri-tumoral sEEG electrodes for peri-operative language mapping and demarcation of the peri-tumoral ictal zone prior to DNET resection. Using electrodes that passed through tumor tissue, we show successful isolation of tumor DNA and subsequent analysis using standard methods for tumor classification by DNA, including Glioseq targeted sequencing and DNA methylation array analysis. This study provides preliminary evidence for the feasibility of molecular diagnosis of LEATs or other lesions using a minimally invasive method with microscopic tissue volumes. The implications of sEEG electrodes in tumor characterization are broad but would aid in diagnosis and subsequent targeted therapeutic strategies.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Encefálicas
/
Epilepsia
Tipo de estudo:
Risk_factors_studies
Limite:
Adolescent
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Child
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Humans
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Male
Idioma:
En
Revista:
Epilepsia Open
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos