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[Cangxi Tongbi Capsules promote chondrocyte autophagy by regulating circRNA_0008365/miR-1271/p38 MAPK pathway to inhibit development of knee osteoarthritis].
Xie, Wen-Peng; Ma, Teng; Liang, Yan-Chen; Wang, Xiang-Peng; Bi, Rong-Xiu; Wang, Wei-Guo; Zhang, Yong-Kui.
Afiliação
  • Xie WP; Department of Orthopaedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine Ji'nan 250014, China.
  • Ma T; Shandong University of Traditional Chinese Medicine Ji'nan 250355,China.
  • Liang YC; Department of Orthopaedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine Ji'nan 250014, China.
  • Wang XP; Department of Orthopaedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine Ji'nan 250014, China Shandong University of Traditional Chinese Medicine Ji'nan 250355,China.
  • Bi RX; Department of Orthopaedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine Ji'nan 250014, China Shandong University of Traditional Chinese Medicine Ji'nan 250355,China.
  • Wang WG; Department of Orthopaedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine Ji'nan 250014, China.
  • Zhang YK; Department of Orthopaedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine Ji'nan 250014, China.
Zhongguo Zhong Yao Za Zhi ; 48(18): 4843-4851, 2023 Sep.
Article em Zh | MEDLINE | ID: mdl-37802826
ABSTRACT
To investigate the mechanism by which Cangxi Tongbi Capsules promote chondrocyte autophagy to inhibit knee osteoarthritis(KOA) progression by regulating the circRNA_0008365/miR-1271/p38 mitogen-activated protein kinase(MAPK) pathway. The cell and animal models of KOA were established and intervened with Cangxi Tongbi Capsules, si-circRNA_0008365, si-NC, and Cangxi Tongbi Capsules combined with si-circRNA_0008365. Flow cytometry and transmission electron microscopy were employed to determine the level of apoptosis and observe autophagosomes, respectively. Western blot was employed to reveal the changes in the protein levels of microtubule-associated protein light chain 3(LC3)Ⅱ/Ⅰ, Beclin-1, selective autophagy junction protein p62/sequestosome 1, collagen Ⅱ, a disintegrin and metalloproteinase with thrombospondin motifs 5(ADAMTS-5), and p38 MAPK. The mRNA levels of circRNA_0008365, miR-1271, collagen Ⅱ, and ADAMTS-5 were determined by qRT-PCR. Hematoxylin-eosin staining was employed to reveal the pathological changes of the cartilage tissue of the knee, and enzyme-linked immunosorbent assay to measure the levels of interleukin-1ß(IL-1ß) and tumor necrosis factor-alpha(TNF-α). The chondrocytes treated with IL-1ß showed down-regulated expression of circRNA_0008365, up-regulated expression of miR-1271 and p38 MAPK, lowered autophagy level, increased apoptosis rate, and accelerated catabolism of extracellular matrix. The intervention with Cangxi Tongbi Capsules up-regulated the expression of circRNA_0008365, down-regulated the expression of miR-1271 and p38 MAPK, increased the autophagy level, decreased the apoptosis rate, and weakened the catabolism of extracellular matrix. However, the effect of Cangxi Tongbi Capsules was suppressed after interfering with circRNA_0008365. The in vivo experiments showed that Cangxi Tongbi Capsules dose-dependently inhibited the p38 MAPK pathway, enhanced chondrocyte autophagy, and mitigated articular cartilage damage and inflammatory response, thereby inhibiting the progression of KOA in rats. This study indicated that Cangxi Tongbi Capsules promoted chondrocyte autophagy by regulating the circRNA_0008365/miR-1271/p38 MAPK pathway to inhibit the development of KOA.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osteoartrite do Joelho / MicroRNAs Tipo de estudo: Prognostic_studies Limite: Animals Idioma: Zh Revista: Zhongguo Zhong Yao Za Zhi Assunto da revista: FARMACOLOGIA / TERAPIAS COMPLEMENTARES Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osteoartrite do Joelho / MicroRNAs Tipo de estudo: Prognostic_studies Limite: Animals Idioma: Zh Revista: Zhongguo Zhong Yao Za Zhi Assunto da revista: FARMACOLOGIA / TERAPIAS COMPLEMENTARES Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China