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Characterization of complete mitochondrial genome of three Horse flies of the genus Tabanus (Diptera: Tabanidae): comparative analysis.
Mukherjee, Arka; Ghosh, Abhishek; Tyagi, Kaomud; Kumar, Vikas; Banerjee, Dhriti; Naskar, Atanu.
Afiliação
  • Mukherjee A; Diptera Section, Zoological Survey of India, Kolkata, West Bengal, India.
  • Ghosh A; Department of Zoology, University of Calcutta, Kolkata, West Bengal, India.
  • Tyagi K; Department of Zoology, University of Calcutta, Kolkata, West Bengal, India.
  • Kumar V; Centre for DNA Taxonomy, Molecular Systematics Division, Zoological Survey of India, Kolkata, West Bengal, India.
  • Banerjee D; Centre for DNA Taxonomy, Molecular Systematics Division, Zoological Survey of India, Kolkata, West Bengal, India. kumud.tyagi5@gmail.com.
  • Naskar A; Centre for DNA Taxonomy, Molecular Systematics Division, Zoological Survey of India, Kolkata, West Bengal, India. vikaszsi77@gmail.com.
Mol Biol Rep ; 50(12): 9897-9908, 2023 Dec.
Article em En | MEDLINE | ID: mdl-37864662
ABSTRACT

BACKGROUND:

Tabanidae (Horse-Flies or Deer-Flies) are one of the most economically important as well as medically significant haematophagous insect family within the order Diptera. Members of this group are also responsible for the mortality of substantial number of live-stock every year. Due to their pathogen transmission potential and vector competencies makes them an important insect group to study. Till now, mitochondrial genome of 18 species of tabanids were available. METHODS AND

RESULTS:

The complete mitogenome of three species T. diversifrons (15,809 bp), T. rubidus (15,878 bp) and T. tenens (15,872 bp) were generated by Next generation sequencing method. They consist 37 genes, with a positive AT skew and a negative GC skew. The gene order of these three species is similar to the typical gene arrangement of infra-order Tabanomorpha. Most of the tRNAs showed typical clover-leaf secondary structure except trnS1, which lacks the DHU arm. The sliding window analysis showed that the nad4L is the most conserved while atp8, and nad6 are the most variable genes. Moreover, the ratios of non-synonymous to synonymous substitution rates indicated that all PCGs under the purifying selection. Phylogeny revealed Chrysops and Haematopota are monophyletic while species of Hybomitra are nested within the polyphyletic clade of Tabanus. T. diversifrons exhibits sister relationship with Atylotus miser. Two morphologically divergent species T. rubidus and T. tenens are found to be genetically similar and indistinguishable by mitochondrial genome.

CONCLUSIONS:

The hypervariable genes like atp8 and nad6 can be used as molecular markers for the identification of recently diverged lineages of family Tabanidae. Further, to address uncertainties arising from the two morphological divergent species, it is imperative to obtain data from nuclear gene markers.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cervos / Dípteros / Genoma Mitocondrial Limite: Animals Idioma: En Revista: Mol Biol Rep Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Índia

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cervos / Dípteros / Genoma Mitocondrial Limite: Animals Idioma: En Revista: Mol Biol Rep Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Índia