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Detection and identification of amino acids and proteins using their intrinsic fluorescence in the visible light spectrum.
Verma, Rajni; Pyreddy, Suneela; Redmond, Connagh E; Qazi, Farah; Khalid, Asma; O'Brien-Simpson, Neil M; Shukla, Ravi; Tomljenovic-Hanic, Snjezana.
Afiliação
  • Verma R; School of Physics, University of Melbourne, Parkville, 3010, Australia; National Creative Research Center for Spin Dynamics and SW Devices, Department of Material Sciences and Engineering, Seoul National University, Seoul 151-744, South Korea. Electronic address: rajnidayal91@gmail.com.
  • Pyreddy S; Sir Ian Potter Biosensing Facility and Nanobiotechnology Research Laboratory, School of Science, RMIT University, Melbourne, Victoria, 3001, Australia; Centre for Advanced Materials & Industrial Chemistry RMIT University, Melbourne, Victoria 3001, Australia.
  • Redmond CE; ACTV Research Group, Oral Health Research Centre, Melbourne Dental School, The University of Melbourne, Melbourne, Victoria, 3010, Australia.
  • Qazi F; School of Physics, University of Melbourne, Parkville, 3010, Australia.
  • Khalid A; School of Science, RMIT University, Melbourne, Victoria, 3001, Australia.
  • O'Brien-Simpson NM; ACTV Research Group, Oral Health Research Centre, Melbourne Dental School, The University of Melbourne, Melbourne, Victoria, 3010, Australia.
  • Shukla R; Sir Ian Potter Biosensing Facility and Nanobiotechnology Research Laboratory, School of Science, RMIT University, Melbourne, Victoria, 3001, Australia; Centre for Advanced Materials & Industrial Chemistry RMIT University, Melbourne, Victoria 3001, Australia.
  • Tomljenovic-Hanic S; School of Physics, University of Melbourne, Parkville, 3010, Australia.
Anal Chim Acta ; 1282: 341925, 2023 Nov 22.
Article em En | MEDLINE | ID: mdl-37923411
The detection and identification of biomolecules are essential in the modern era of medical diagnostics. Several approaches have been established, but they have significant limitations such as laborious and time-consuming sample preparation, analysis, and the need to use external probes which provide adequate but not desired levels of accuracy and sensitivity. Herein, we have explored successfully a non-invasive technique to detect and identifybiomolecules such as amino acids and proteins by utilizing their intrinsic fluorescence. The developed confocal microscopy method revealed high and photostable emission counts of these biomolecules including amino acids (tryptophan, phenylalanine, tyrosine, proline, histidine, cysteine, aspartic acid, asparagine, isoleucine, lysine, glutamic acid, arginine) and proteins (HSA, BSA) when they are excited with a green laser. The fluorescence lifetime of the samples enabled the identification and distinction of known and blind samples of biomolecules from each other. The developed optical technique is straightforward, non-destructive and does not require laborious labeling to identify specific proteins, and may serve as the basis for the development of a device that would quickly and accurately identify proteins at an amino acid level. Therefore, this approach would open an avenue for precise detection in imaging and at the same time increases our understanding of chemical dynamics at the molecular level.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Alanina / Aminoácidos Idioma: En Revista: Anal Chim Acta Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Alanina / Aminoácidos Idioma: En Revista: Anal Chim Acta Ano de publicação: 2023 Tipo de documento: Article