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3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex.
Vue, Zer; Garza-Lopez, Edgar; Neikirk, Kit; Katti, Prasanna; Vang, Larry; Beasley, Heather; Shao, Jianqiang; Marshall, Andrea G; Crabtree, Amber; Murphy, Alexandria C; Jenkins, Brenita C; Prasad, Praveena; Evans, Chantell; Taylor, Brittany; Mungai, Margaret; Killion, Mason; Stephens, Dominique; Christensen, Trace A; Lam, Jacob; Rodriguez, Benjamin; Phillips, Mark A; Daneshgar, Nastaran; Koh, Ho-Jin; Koh, Alice; Davis, Jamaine; Devine, Nina; Saleem, Mohammad; Scudese, Estevão; Arnold, Kenneth Ryan; Vanessa Chavarin, Valeria; Daniel Robinson, Ryan; Chakraborty, Moumita; Gaddy, Jennifer A; Sweetwyne, Mariya T; Wilson, Genesis; Zaganjor, Elma; Kezos, James; Dondi, Cristiana; Reddy, Anilkumar K; Glancy, Brian; Kirabo, Annet; Quintana, Anita M; Dai, Dao-Fu; Ocorr, Karen; Murray, Sandra A; Damo, Steven M; Exil, Vernat; Riggs, Blake; Mobley, Bret C; Gomez, Jose A.
Afiliação
  • Vue Z; Department of Molecular Physiology and Biophysics, Vanderbilt University, Tennessee, Nashville, USA.
  • Garza-Lopez E; Department of Internal Medicine, University of Iowa, Iowa, Iowa City, USA.
  • Neikirk K; Department of Molecular Physiology and Biophysics, Vanderbilt University, Tennessee, Nashville, USA.
  • Katti P; National Heart, Lung and Blood Institute, National Institutes of Health, Maryland, Bethesda, USA.
  • Vang L; Department of Molecular Physiology and Biophysics, Vanderbilt University, Tennessee, Nashville, USA.
  • Beasley H; Department of Molecular Physiology and Biophysics, Vanderbilt University, Tennessee, Nashville, USA.
  • Shao J; Central Microscopy Research Facility, University of Iowa, Iowa, Iowa City, USA.
  • Marshall AG; Department of Molecular Physiology and Biophysics, Vanderbilt University, Tennessee, Nashville, USA.
  • Crabtree A; Department of Molecular Physiology and Biophysics, Vanderbilt University, Tennessee, Nashville, USA.
  • Murphy AC; Department of Biochemistry and Molecular Biology, The Huck Institute of the Life Sciences, Pennsylvania State University, Pennsylvania, State College, USA.
  • Jenkins BC; Department of Biochemistry and Molecular Biology, The Huck Institute of the Life Sciences, Pennsylvania State University, Pennsylvania, State College, USA.
  • Prasad P; Department of Biochemistry and Molecular Biology, The Huck Institute of the Life Sciences, Pennsylvania State University, Pennsylvania, State College, USA.
  • Evans C; Department of Cell Biology, Duke University School of Medicine, North Carolina, Durham, USA.
  • Taylor B; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Florida, Gainesville, USA.
  • Mungai M; Department of Molecular Physiology and Biophysics, Vanderbilt University, Tennessee, Nashville, USA.
  • Killion M; Department of Molecular Physiology and Biophysics, Vanderbilt University, Tennessee, Nashville, USA.
  • Stephens D; Department of Molecular Physiology and Biophysics, Vanderbilt University, Tennessee, Nashville, USA.
  • Christensen TA; Microscopy and Cell Analysis Core Facility, Mayo Clinic, Minnesota, Rochester, USA.
  • Lam J; Department of Internal Medicine, University of Iowa, Iowa, Iowa City, USA.
  • Rodriguez B; Department of Internal Medicine, University of Iowa, Iowa, Iowa City, USA.
  • Phillips MA; Department of Integrative Biology, Oregon State University, Oregon, Corvallis, USA.
  • Daneshgar N; Department of Integrative Biology, Oregon State University, Oregon, Corvallis, USA.
  • Koh HJ; Department of Biological Sciences, Tennessee State University, Tennessee, Nashville, USA.
  • Koh A; Department of Molecular Physiology and Biophysics, Vanderbilt University, Tennessee, Nashville, USA.
  • Davis J; Department of Medicine, Vanderbilt University Medical Center, Tennessee, Nashville, USA.
  • Devine N; Department of Biochemistry, Cancer Biology, Neuroscience, and Pharmacology, Meharry Medical College, Tennessee, Nashville, USA.
  • Saleem M; Department of Integrative Biology, Oregon State University, Oregon, Corvallis, USA.
  • Scudese E; Department of Medicine, Vanderbilt University Medical Center, Tennessee, Nashville, USA.
  • Arnold KR; Laboratory of Biosciences of Human Motricity (LABIMH) of the Federal University of State of Rio de Janeiro (UNIRIO), Rio de Janeiro, Brazil.
  • Vanessa Chavarin V; Sport Sciences and Exercise Laboratory (LaCEE), Catholic University of Petrópolis (UCP), Petrópolis, State of Rio de Janeiro, Brazil.
  • Daniel Robinson R; Department of Ecology and Evolutionary Biology, University of California at Irvine, California, Irvine, USA.
  • Chakraborty M; Department of Ecology and Evolutionary Biology, University of California at Irvine, California, Irvine, USA.
  • Gaddy JA; Department of Ecology and Evolutionary Biology, University of California at Irvine, California, Irvine, USA.
  • Sweetwyne MT; Department of Integrative Biology, Oregon State University, Oregon, Corvallis, USA.
  • Wilson G; Department of Molecular Physiology and Biophysics, Vanderbilt University, Tennessee, Nashville, USA.
  • Zaganjor E; Department of Medicine, Vanderbilt University Medical Center, Tennessee, Nashville, USA.
  • Kezos J; Department of Medicine Health and Society, Vanderbilt University, Tennessee, Nashville, USA.
  • Dondi C; Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Tennessee, Nashville, USA.
  • Reddy AK; Department of Veterans Affairs, Tennessee Valley Healthcare Systems, Tennessee, Nashville, USA.
  • Glancy B; Department of Laboratory Medicine and Pathology, University of Washington, Washington, Seattle, USA.
  • Kirabo A; Department of Molecular Physiology and Biophysics, Vanderbilt University, Tennessee, Nashville, USA.
  • Quintana AM; Department of Molecular Physiology and Biophysics, Vanderbilt University, Tennessee, Nashville, USA.
  • Dai DF; Sanford Burnham Prebys Medical Discovery Institute, California, La Jolla, USA.
  • Ocorr K; Sanford Burnham Prebys Medical Discovery Institute, California, La Jolla, USA.
  • Murray SA; Department of Medicine, Baylor College of Medicine, Texas, Houston, USA.
  • Damo SM; National Heart, Lung and Blood Institute, National Institutes of Health, Maryland, Bethesda, USA.
  • Exil V; National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Maryland, Bethesda, USA.
  • Riggs B; Department of Molecular Physiology and Biophysics, Vanderbilt University, Tennessee, Nashville, USA.
  • Mobley BC; Department of Medicine, Vanderbilt University Medical Center, Tennessee, Nashville, USA.
  • Gomez JA; Department of Biological Sciences, Border Biomedical Research Center, University of Texas at El Paso, Texas, El Paso, USA.
Aging Cell ; 22(12): e14009, 2023 Dec.
Article em En | MEDLINE | ID: mdl-37960952
During aging, muscle gradually undergoes sarcopenia, the loss of function associated with loss of mass, strength, endurance, and oxidative capacity. However, the 3D structural alterations of mitochondria associated with aging in skeletal muscle and cardiac tissues are not well described. Although mitochondrial aging is associated with decreased mitochondrial capacity, the genes responsible for the morphological changes in mitochondria during aging are poorly characterized. We measured changes in mitochondrial morphology in aged murine gastrocnemius, soleus, and cardiac tissues using serial block-face scanning electron microscopy and 3D reconstructions. We also used reverse transcriptase-quantitative PCR, transmission electron microscopy quantification, Seahorse analysis, and metabolomics and lipidomics to measure changes in mitochondrial morphology and function after loss of mitochondria contact site and cristae organizing system (MICOS) complex genes, Chchd3, Chchd6, and Mitofilin. We identified significant changes in mitochondrial size in aged murine gastrocnemius, soleus, and cardiac tissues. We found that both age-related loss of the MICOS complex and knockouts of MICOS genes in mice altered mitochondrial morphology. Given the critical role of mitochondria in maintaining cellular metabolism, we characterized the metabolomes and lipidomes of young and aged mouse tissues, which showed profound alterations consistent with changes in membrane integrity, supporting our observations of age-related changes in muscle tissues. We found a relationship between changes in the MICOS complex and aging. Thus, it is important to understand the mechanisms that underlie the tissue-dependent 3D mitochondrial phenotypic changes that occur in aging and the evolutionary conservation of these mechanisms between Drosophila and mammals.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Imageamento Tridimensional / Membranas Associadas à Mitocôndria Limite: Animals Idioma: En Revista: Aging Cell Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Imageamento Tridimensional / Membranas Associadas à Mitocôndria Limite: Animals Idioma: En Revista: Aging Cell Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos