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Potato virus Y viral protein 6K1 inhibits the interaction between defense proteins during virus infection.
Fang, Le; Geng, Chao; Wei, Xin-Yu; Dong, Chen-Chen; Pang, Ju-Ping; Yan, Zhi-Yong; Jiang, Jun; Tian, Yan-Ping; Li, Xiang-Dong.
Afiliação
  • Fang L; Shandong Provincial Key Laboratory of Agricultural Microbiology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
  • Geng C; Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250131, China.
  • Wei XY; Shandong Provincial Key Laboratory of Agricultural Microbiology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
  • Dong CC; Shandong Provincial Key Laboratory of Agricultural Microbiology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
  • Pang JP; Shandong Provincial Key Laboratory of Agricultural Microbiology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
  • Yan ZY; Shandong Provincial Key Laboratory of Agricultural Microbiology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
  • Jiang J; Shandong Provincial Key Laboratory of Agricultural Microbiology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
  • Tian YP; Shandong Provincial Key Laboratory of Agricultural Microbiology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
  • Li XD; Shandong Provincial Key Laboratory of Agricultural Microbiology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
Plant Physiol ; 194(3): 1447-1466, 2024 Feb 29.
Article em En | MEDLINE | ID: mdl-37962935
14-3-3 proteins play vital roles in plant defense against various pathogen invasions. To date, how 14-3-3 affects virus infections in plants remains largely unclear. In this study, we found that Nicotiana benthamiana 14-3-3h interacts with TRANSLATIONALLY CONTROLLED TUMOR PROTEIN (TCTP), a susceptibility factor of potato virus Y (PVY). Silencing of Nb14-3-3h facilitates PVY accumulation, whereas overexpression of Nb14-3-3h inhibits PVY replication. The antiviral activities of 3 Nb14-3-3h dimerization defective mutants are significantly decreased, indicating that dimerization of Nb14-3-3h is indispensable for restricting PVY infection. Our results also showed that the mutant Nb14-3-3hE16A, which is capable of dimerizing but not interacting with NbTCTP, has reduced anti-PVY activity; the mutant NbTCTPI65A, which is unable to interact with Nb14-3-3h, facilitates PVY replication compared with the wild-type NbTCTP, indicating that dimeric Nb14-3-3h restricts PVY infection by interacting with NbTCTP and preventing its proviral function. As a counter-defense, PVY 6K1 interferes with the interaction between Nb14-3-3h and NbTCTP by competitively binding to Nb14-3-3h and rescues NbTCTP to promote PVY infection. Our results provide insights into the arms race between plants and potyviruses.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Viroses / Potyvirus Limite: Humans Idioma: En Revista: Plant Physiol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Viroses / Potyvirus Limite: Humans Idioma: En Revista: Plant Physiol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China