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Staphylococcus aureus lysate induces an IgE response via memory B cells in nasal polyps.
Du, Kun; Zhao, Yan; Zhang, Xin; Li, Chenduo; Hao, Yun; Du, Xiaonan; Yang, Yiran; Qin, Xiaofeng; Hu, Yue; Li, Ying; Wang, Yue; Chen, Yan; Li, Yan; Wang, Wei; Wang, Xiangdong; Ying, Sun; Zhang, Luo.
Afiliação
  • Du K; Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China; Department of Otolaryngology-Head and Neck Surgery, Eye & ENT Hospital, Fudan University, Shanghai, China.
  • Zhao Y; Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China; Beijing Laboratory of Allergic Diseases, Beijing Municipal Education Commission and Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China.
  • Zhang X; Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing, China.
  • Li C; Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing, China.
  • Hao Y; Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
  • Du X; Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing, China.
  • Yang Y; Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing, China.
  • Qin X; Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing, China.
  • Hu Y; Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing, China.
  • Li Y; Beijing Laboratory of Allergic Diseases, Beijing Municipal Education Commission and Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China.
  • Wang Y; Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
  • Chen Y; Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing, China.
  • Li Y; Beijing Laboratory of Allergic Diseases, Beijing Municipal Education Commission and Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China.
  • Wang W; Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing, China.
  • Wang X; Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China; Beijing Laboratory of Allergic Diseases, Beijing Municipal Education Commission and Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China.
  • Ying S; Beijing Laboratory of Allergic Diseases, Beijing Municipal Education Commission and Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China; Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing, China. Electronic addres
  • Zhang L; Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China; Beijing Laboratory of Allergic Diseases, Beijing Municipal Education Commission and Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China;
J Allergy Clin Immunol ; 153(3): 718-731.e11, 2024 Mar.
Article em En | MEDLINE | ID: mdl-38056634
ABSTRACT

BACKGROUND:

Locally increased IgE levels plays a pathologic role in chronic rhinosinusitis with nasal polyps (CRSwNP).

OBJECTIVE:

This study aimed to investigate whether Staphylococcus aureus could induce aberrant IgE synthesis in CRSwNP and the potential mechanisms involved.

METHODS:

Total IgE, IL-4, IL-5, and IL-13 concentrations in the supernatants of the cultures stimulated with S aureus lysate were assessed by ELISA. S aureus-induced cellular responses were investigated by single-cell RNA sequencing. Flow cytometry and quantitative reverse transcription PCR were used to analyze B-cell subsets and stimulated cell ε-germline transcript expression, respectively. IgE-positive B-cell and germinal center localization were assessed by immunohistochemistry and immunofluorescence.

RESULTS:

S aureus lysate induced IgE production in the supernatants of nasal polyp (NP) tissues but not in those of healthy nasal mucosa. Moreover, IgE levels increased from days 2 to 4 after stimulation, paralleling the enhanced ε-germline transcript, IL-5, and IL-13 expression. Single-cell RNA sequencing revealed that there were increased IL-5 and IL-13 in group 2 innate lymphoid cells and identified a clonal overlap between unstimulated memory B cells and S aureus-stimulated plasma cells. The enriched IgE within NPs was mainly produced by IgE-negative memory B cells. Cellular evidence indicated that the IgE memory response to S aureus might also exist in the peripheral blood of CRSwNP patients. The S aureus-induced IgE memory response was associated with elevated IgE levels in NPs, asthma, and postoperative CRSwNP recurrence.

CONCLUSIONS:

S aureus induced an IgE response via IgE-negative memory B cells in CRSwNP patients, possibly contributing to CRSwNP development.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sinusite / Rinite / Pólipos Nasais Limite: Humans Idioma: En Revista: J Allergy Clin Immunol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sinusite / Rinite / Pólipos Nasais Limite: Humans Idioma: En Revista: J Allergy Clin Immunol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China