Alanine aminotransferase electrochemical sensor based on graphene@MXene composite nanomaterials.
Mikrochim Acta
; 191(1): 45, 2023 12 19.
Article
em En
| MEDLINE
| ID: mdl-38114837
ABSTRACT
Graphene@MXene composite nanomaterials were utilized to construct an electrochemical sensor for alanine aminotransferase (ALT) detection. The combination of graphene nanosheets with MXene avoids the self-stacking of MXene and graphene, and broadens the charge transfer channel. In addition, the composite nanomaterial provides increased loading sites for pyruvate oxidase. The principle of ALT detection is a two-step enzymatic reaction. L-Alanine was initially transferred to pyruvate catalyzed by ALT. The formed pyruvate was then oxidized by pyruvate oxidase, generating H2O2. Through the detection of the generated H2O2, ALT activity was measured. The linear range of the sensor to ALT was from 5 to 400 U·L-1 with a detection limit of 0.16 U·L-1 (S/N = 3). For real sample analysis, the spiked recovery test results of ALT in serum samples were between 96.89 and 103.93% with RSD < 5%, confirming the reliability of the sensor testing results and potential clinical application of the sensor.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Nanoestruturas
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Grafite
Idioma:
En
Revista:
Mikrochim Acta
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
China