Theoretical computation of the band broadening in micro-pillar array columns.
J Chromatogr A
; 1715: 464607, 2024 Jan 25.
Article
em En
| MEDLINE
| ID: mdl-38154258
ABSTRACT
We have investigated the possibility to establish a theoretical plate height expression for the band broadening in the most widely used micro-pillar array column format, i.e., a cylindrical pillar array wherein the pillar walls and the channel bottom are coated with a thin layer of mesoporous material. Assuming isotropic diffusion in the shell-layer, it was found that the vertical diffusive transport along the porous shell-layer covering the pillar walls significantly suppresses the band broadening originating from the vertical migration velocity gradients. As the vertical transport in the shell-layer increases linearly with the retention equilibrium constant K, this leads to an anomalous dependency on the retention factor. Indeed, instead of increasing with k'' and following the classic (1+ak''+bk''2)/(1 + k'')2-dependency governing a classic Taylor-Aris system, the variation of the mobile zone mass transfer resistance term hCm in a 3D pillar array with bottom-wall retention goes through a maximum (resp. factor 1.5 (k''=4) and 2 (k''=16) difference between observed and classic Taylor-Aris behaviour). This effect increases with increasing pillar heights and increasing reduced velocities. Because of this complex k''-dependency, it proves very cumbersome to establish a general plate height equation covering all conditions. Instead, a plate height expression was established that is limited up to k''=4, but remains accurate for higher k''-values for cases where the ratio of pillar height over inter-pillar distance remains below 5. It can however be anticipated the proposed analytical model is only valid in a rather limited range around the presently considered external porosity of ε=0.5.
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MEDLINE
Assunto principal:
Porosidade
Idioma:
En
Revista:
J Chromatogr A
/
J. chromatogr. A
/
Journal of chromatography A
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Bélgica