Development of an activatable Lysosome-targeted fluorescent probe for the detection of endogenous ONOO- levels.
Talanta
; 270: 125581, 2024 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-38159352
ABSTRACT
The liver plays a crucial role in several important processes in the human body, including metabolism, detoxification, and immune function. When the liver experiences acute injury, it can cause significant harm and requires prompt detection. Traditional biomarkers lack specificity and cannot detect changes in real-time, making them unsuitable for monitoring pathological processes. Recent studies have shown that acute liver injury (ALI) is closely related to oxidative stress, with peroxynitrite (ONOO-) being a vital byproduct of liver metabolism and become a critical biomarker for detecting liver damage. As a result, this research developed an activatable near-infrared fluorescent probe W-3a that can be used to detect endogenous ONOO- in a mouse model of ALI induced by lipopolysaccharides (LPS). The probe has high selectivity and anti-interference ability, with a reaction time <10 min and a detection limit of 85 nM. It was successfully utilized in detecting endogenous ONOO- in cells and live imaging of ALI mice.
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Base de dados:
MEDLINE
Assunto principal:
Corantes Fluorescentes
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Fígado
Limite:
Animals
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Humans
Idioma:
En
Revista:
Talanta
Ano de publicação:
2024
Tipo de documento:
Article