Yap/Taz activity is associated with increased expression of phosphoglycerate dehydrogenase that supports myoblast proliferation.
Cell Tissue Res
; 395(3): 271-283, 2024 Mar.
Article
em En
| MEDLINE
| ID: mdl-38183459
ABSTRACT
In skeletal muscle, the Hippo effector Yap promotes satellite cell, myoblast, and rhabdomyoblast proliferation but prevents myogenic differentiation into multinucleated muscle fibres. We previously noted that Yap drives expression of the first enzyme of the serine biosynthesis pathway, phosphoglycerate dehydrogenase (Phgdh). Here, we examined the regulation and function of Phgdh in satellite cells and myoblasts and found that Phgdh protein increased during satellite cell activation. Analysis of published data reveal that Phgdh mRNA in mouse tibialis anterior muscle was highly expressed at day 3 of regeneration after cardiotoxin injection, when markers of proliferation are also robustly expressed and in the first week of synergist-ablated muscle. Finally, siRNA-mediated knockdown of PHGDH significantly reduced myoblast numbers and the proliferation rate. Collectively, our data suggest that Phgdh is a proliferation-enhancing metabolic enzyme that is induced when quiescent satellite cells become activated.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células Satélites de Músculo Esquelético
/
Fosfoglicerato Desidrogenase
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Cell Tissue Res
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Alemanha