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LINC01234 promoted malignant behaviors of breast cancer cells via hsa-miR-30c-2-3p/CCT4/mTOR signaling pathway.
Tang, Chuangang; Li, Changwen; Chen, Chengling; Chen, Tao; Zhu, Juan; Sun, Mingyu; Wang, Pei; Han, Conghui.
Afiliação
  • Tang C; Suzhou Medical College of Soochow University, Suzhou, Jiangsu, 215123, China; Department of Breast Surgery, Xuzhou Central Hospital, Postgraduate Workstation of Soochow University Xuzhou, Jiangsu, 221009, China.
  • Li C; Department of Breast Surgery, Xuzhou Central Hospital, Jiangsu, 221009, China.
  • Chen C; Department of Breast Surgery, Xuzhou Central Hospital, Jiangsu, 221009, China.
  • Chen T; The Xuzhou Clinical College of Xuzhou Medical University, Jiangsu, 221009, China.
  • Zhu J; Department of Breast Surgery, Xuzhou Central Hospital, Jiangsu, 221009, China.
  • Sun M; Department of Breast Surgery, Xuzhou Central Hospital, Jiangsu, 221009, China. Electronic address: 645396517@qq.com.
  • Wang P; Department of Breast Surgery, Xuzhou Central Hospital, Jiangsu, 221009, China. Electronic address: 1349533289@qq.com.
  • Han C; Suzhou Medical College of Soochow University, Suzhou, Jiangsu, 215123, China; Department of Urology, Xuzhou Central Hospital, Xuzhou, Jiangsu, 221009, China; Department of Urology, Xuzhou Clinical School of Xuzhou Medical University, Jiangsu, 221009, China; Department of Urology, Heilongjiang Provin
Taiwan J Obstet Gynecol ; 63(1): 46-56, 2024 Jan.
Article em En | MEDLINE | ID: mdl-38216268
ABSTRACT

OBJECTIVE:

Despite continuous progress in treatment, recurrence and metastasis limit further improvement in the prognosis of breast cancer (BC) patients. Our aim was to search for a crucial prognostic biomarker of BC. MATERIALS AND

METHODS:

Patient data were selected from The Cancer Genome Atlas (TCGA) and GTEx databases. Several online public databases, including Gene Expression Profiling Interactive Analysis (GEPIA), miRWalk, miRDB, and LncBase Predicted v.2, were used to identify potential upstream miRNAs and lncRNAs. These findings were validated through in vitro experiments.

RESULTS:

A total of 1, 097 invasive BC samples and 572 normal breast tissues (including 113 samples from TCGA and 459 samples from GTEx) were collected for the study. CCT4 was not only significantly overexpressed in BC compared with normal breast tissues but also had important prognostic significance (P < 0.001). By intersecting miRWalk and miRDB and conducting correlation analysis, hsa-miR-30c-2-3p was identified as the most probable upstream miRNA of CCT4. Following an extensive assessment that included survival analysis, correlation analysis, and common binding-site prediction, LINC01234 was chosen as the most likely upstream lncRNA. In vitro experiments showed that LINC01234-siRNA inhibited the proliferation, invasion, and migration abilities of BC cells. Western blot analysis further confirmed that LINC01234 promoted malignant behaviors of BC cells via the CCT4/mTOR signaling pathway.

CONCLUSION:

The LINC01234/hsa-miR-30c-2-3p/CCT4/mTOR axis was identified as a potential ceRNA regulatory mechanism in BC. These findings established the foundation for systematically unveiling the pathological mechanisms of BC and provided new insights for targeted therapy of BC patients.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / MicroRNAs / RNA Longo não Codificante Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Revista: Taiwan J Obstet Gynecol Assunto da revista: GINECOLOGIA / OBSTETRICIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / MicroRNAs / RNA Longo não Codificante Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Revista: Taiwan J Obstet Gynecol Assunto da revista: GINECOLOGIA / OBSTETRICIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China