Single source CARS-based multimodal microscopy system for biological tissue imaging [Invited].
Biomed Opt Express
; 15(1): 131-141, 2024 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-38223172
ABSTRACT
A coherent anti-Stokes Raman scattering (CARS)-based multimodality microscopy system was developed using a single Tisapphire femtosecond laser source for biological imaging. It provides three complementary and co-registered imaging modalities CARS, MPM (multiphoton microscopy), and RCM (reflectance confocal microscopy). The imaging speed is about 1 frame-per-second (fps) with a digital resolution of 1024 × 1024 pixels. This microscopy system can provide clear 2-dimensional and 3-dimensional images of ex-vivo biological tissue samples. Its spectral selection initiates vibrational excitation in lipid cells (approximately 2850â
cm-1) using two filters on the pump and Stokes beam paths. The excitation can be tuned over a wide spectral range with adjustable spectral filters. The imaging capability of this CARS-based multimodal microscopy system was demonstrated using porcine fat, murine skin, and murine liver tissue samples.
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Base de dados:
MEDLINE
Idioma:
En
Revista:
Biomed Opt Express
/
Biomedical optics express
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Canadá