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Tissue engineering RPE sheet derived from hiPSC-RPE cell spheroids supplemented with Y-27632 and RepSox.
Wang, Wenxuan; Yang, Tingting; Chen, Sihui; Liang, Liying; Wang, Yingxin; Ding, Yin; Xiong, Wei; Ye, Xiuhong; Guo, Yonglong; Shen, Shuhao; Chen, Hang; Chen, Jiansu.
Afiliação
  • Wang W; Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
  • Yang T; Institute of Ophthalmology, Medical College, Jinan University, Guangzhou, China.
  • Chen S; Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
  • Liang L; Institute of Ophthalmology, Medical College, Jinan University, Guangzhou, China.
  • Wang Y; Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
  • Ding Y; Institute of Ophthalmology, Medical College, Jinan University, Guangzhou, China.
  • Xiong W; Institute of Ophthalmology, Medical College, Jinan University, Guangzhou, China.
  • Ye X; Institute of Ophthalmology, Medical College, Jinan University, Guangzhou, China.
  • Guo Y; The University of Hong Kong - Shenzhen Hospital, Shenzhen, China.
  • Shen S; Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou, China.
  • Chen H; Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
  • Chen J; Institute of Ophthalmology, Medical College, Jinan University, Guangzhou, China.
J Biol Eng ; 18(1): 7, 2024 Jan 16.
Article em En | MEDLINE | ID: mdl-38229139
ABSTRACT

BACKGROUND:

Retinal pigment epithelium (RPE) cell therapy is a promising way to treat many retinal diseases. However, obtaining transplantable RPE cells is time-consuming and less effective. This study aimed to develop novel strategies for generating engineered RPE patches with physiological characteristics.

RESULTS:

Our findings revealed that RPE cells derived from human induced pluripotent stem cells (hiPSCs) successfully self-assembled into spheroids. The RPE spheroids treated with Y27632 and Repsox had increased expression of epithelial markers and RPE-specific genes, along with improved cell viability and barrier function. Transcriptome analysis indicated enhanced cell adhesion and extracellular matrix (ECM) organization in RPE spheroids. These RPE spheroids could be seeded and bioprinted on collagen vitrigel (CV) membranes to construct engineered RPE sheets. Circular RPE patches, obtained by trephining a specific section of the RPE sheet, exhibited abundant microvilli and pigment particles, as well as reduced proliferative capacity and enhanced maturation.

CONCLUSIONS:

Our study suggests that the supplementation of small molecules and 3D spheroid culture, as well as the bioprinting technique, can be effective methods to promote RPE cultivation and construct engineered RPE sheets, which may support future clinical RPE cell therapy and the development of RPE models for research applications.
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Texto completo: 1 Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: J Biol Eng Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: J Biol Eng Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China