Imaging of Extravasation of Splenocytes in the Dorsal Skinfold Window Chamber.
Methods Mol Biol
; 2773: 137-155, 2024.
Article
em En
| MEDLINE
| ID: mdl-38236543
ABSTRACT
Infiltration of immune cells into the tumor is one of the major drivers of antitumor immune response, which can direct the outcome of anticancer therapies. In mice, implantation of dorsal skinfold window chamber (DSWC) combined with intravital confocal fluorescence microscopy allows real-time observation of splenocyte extravasation and infiltration into tumors. Here, we describe a detailed procedure of the DSWC implantation, splenocyte isolation and fluorescent labeling, intravenous injection of labeled splenocytes, and imaging of splenocyte extravasation into tumors using confocal fluorescence microscopy.
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Base de dados:
MEDLINE
Assunto principal:
Baço
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Neoplasias
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Eslovênia