Deciphering the molecular symphony: Unraveling endothelial-to-mesenchymal transition in corneal endothelial cells.
Exp Eye Res
; 240: 109795, 2024 Mar.
Article
em En
| MEDLINE
| ID: mdl-38253308
ABSTRACT
Understanding the molecular complexity of this phenomenon provides innovative targets for maintaining phenotypic integrity during in vitro expansion, thereby advancing corneal endothelial tissue engineering. In this study, we established an in vitro model to simulate endothelial-to-mesenchymal transition (EndMT) in corneal endothelial cells. Through RNA sequencing, we identified 452 upregulated and 163 downregulated genes, resulting in a total of 615 differentially expressed genes. Key pathways enriched by GO and KEGG analysis include extracellular matrix (ECM) regulation and the PI3K-Akt signaling pathway. Potential hub proteins such as THBS1, ITGA5, COL1A1, and SNAI1/2 were also identified, and their dynamic changes at different time points (0, 2, 12, 24 h) were monitored. Uncovering these key pathways and genes may deepen our understanding of the mechanisms underlying EndMT in corneal endothelial cells, providing valuable insights for optimizing in vitro cultivation strategies.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosfatidilinositol 3-Quinases
/
Células Endoteliais
Idioma:
En
Revista:
Exp Eye Res
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China