Isolation and target identification of anti-renal fibrosis compounds from Cordyceps militaris.
Bioorg Chem
; 144: 107169, 2024 Mar.
Article
em En
| MEDLINE
| ID: mdl-38330722
ABSTRACT
Four undescribed compounds including one aromatic glucoside derivative, cordyceglycoside A (1), one new isoleucine derivative inner salt, cordycepisosalt A (2), a rare four-membered lactam, cinerealactam B (3), and one sesquiterpene derivative, cordycepsetp A (4), together with six known compounds were isolated from Cordyceps militaris. The structures including absolute configurations of these new compounds, were unambiguously elucidated by spectroscopic data analysis and single crystal X-ray diffraction. Biological evaluation of compounds 1-4 showed that 3 displays anti-renal fibrotic activities in TGF-ß1 induced NRK-52e cells. Furthermore, DARTS coupled with LC-MS/MS analysis was used to identify candidate target proteins for 3. Subsequently, C1qbp knockdown using siRNA allowed us to validate the target protein of 3.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cordyceps
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Bioorg Chem
Ano de publicação:
2024
Tipo de documento:
Article