Antibiofilm and antivirulence activities of laminarin-gold nanoparticles in standard and host-mimicking media.
Appl Microbiol Biotechnol
; 108(1): 203, 2024 Feb 13.
Article
em En
| MEDLINE
| ID: mdl-38349556
ABSTRACT
The rapidly rising antimicrobial resistance (AMR) in pathogenic bacteria has become one of the most serious public health challenges, with a high death rate. Most pathogenic bacteria have been recognized as a source of AMR and a primary barrier to antimicrobial treatment failure due to the development of biofilms and the production of virulence factors. In this work, nanotechnology was employed as a substitute method to control the formation of biofilms and attenuate virulence features in Pseudomonas aeruginosa and Staphylococcus aureus. We synthesized biocompatible gold nanoparticles from marine-derived laminarin as potential biofilm and virulence treatments. Laminarin-gold nanoparticles (Lam-AuNPs) have been identified as spherical, 49.84 ± 7.32 nm in size and - 26.49 ± 1.29 mV zeta potential. The MIC value of Lam-AuNPs against several drug-resistant microbial pathogens varied from 2 to 1024 µg/mL in both standard and host-mimicking media. Sub-MIC values of Lam-AuNPs were reported to effectively reduce the production of P. aeruginosa and S. aureus biofilms in both standard and host-mimicking growth media. Furthermore, the sub-MIC of Lam-AuNPs strongly reduced hemolysis, pyocyanin, pyoverdine, protease, and several forms of flagellar and pili-mediated motility in P. aeruginosa. Lam-AuNPs also inhibited S. aureus hemolysis and the production of amyloid fibrils. The Lam-AuNPs strongly dispersed the preformed mature biofilm of these pathogens in a dose-dependent manner. The Lam-AuNPs would be considered an alternative antibiofilm and antivirulence agent to control P. aeruginosa and S. aureus infections. KEY POINTS ⢠Lam-AuNPs were biosynthesized to control biofilm and virulence. ⢠Lam-AuNPs show effective biofilm inhibition in standard and host-mimicking media. ⢠Lam-AuNPs suppress various virulence factors of P. aeruginosa and S. aureus.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nanopartículas Metálicas
/
Glucanos
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Anti-Infecciosos
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Ano de publicação:
2024
Tipo de documento:
Article