Photoacoustic expansion microscopy of melanosomes.
Opt Lett
; 49(4): 798-801, 2024 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-38359185
ABSTRACT
Optical resolution photoacoustic microscopy (OR-PAM) is a hybrid imaging method for visualizing organelles due to the high spatial resolution and abundant optical contrast. Usually, OR-PAM employs high numerical aperture (NA) objectives and high-frequency ultrasonic detectors to resolve three-dimensional (3D) microstructures of cells. Expansion microscopy (ExM) provides a nanoscale resolution by isotropically enlarging cells instead of utilizing ultrahigh NA objectives. In this Letter, we report the development of photoacoustic expansion microscopy (PA-ExM) that combines the advantages of OR-PAM and ExM for 3D organelle imaging using near-infrared light. We evaluate the performance of PA-ExM using label-free melanoma cells, where the image quality of melanosome distributions in expanded cells using a 40× objective is comparable to that of unexpanded cells using an oil-immersed 100× objective. The results suggest that PA-ExM possesses the great potential to study organelles.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Fotoacústicas
/
Microscopia
Idioma:
En
Revista:
Opt Lett
Ano de publicação:
2024
Tipo de documento:
Article