The basic chemical substances of total alkaloids of Uncaria rhynchophylla and their anti-neuroinflammatory activities.
J Asian Nat Prod Res
; 26(6): 765-771, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38373226
ABSTRACT
To clarify the chemical basis of the total alkaloids of Uncaria rhynchophylla, HPLC-VWD chromatogram of total alkaloids was established. Under its guidance, modern chromatographic and spectroscopic techniques were used to track, isolate and identify the representative principal components. As a result, one new monoterpenoid indole alkaloid, 3S,15S-N4-methoxymethyl-geissoschizine methyl ether (1), together with 20 known alkaloids (2-21), and 5 other known compounds (22-26) were obtained. Meanwhile, sixteen characteristic peaks were identified from the total alkaloids using HPLC analysis. Then, the anti-neuroinflammatory effect of compounds 1-21 was assessed through inhibiting nitric ---oxide (NO) production in lipopolysaccharide (LPS)-induced BV-2 microglial cells. Among them, compounds 1, 3, 7, 8, 11, 12, 19 and 21 showed potent inhibitory activities with IC50 values of 5.87-76.78 µM.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Lipopolissacarídeos
/
Microglia
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Alcaloides Indólicos
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Uncaria
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Alcaloides
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Anti-Inflamatórios
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Óxido Nítrico
Limite:
Animals
Idioma:
En
Revista:
J Asian Nat Prod Res
Assunto da revista:
BOTANICA
/
QUIMICA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China