Protocol for induction of human kidney cell fibrosis.
STAR Protoc
; 5(1): 102881, 2024 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-38386546
ABSTRACT
Here, we present a protocol for inducing fibrosis in human kidney-2 (HK2) cells followed by quantitative real-time PCR analysis of fibrosis-related genes. We describe steps for growing and expanding cells, inducing HK2 fibrosis, and collecting cells for downstream applications. Given the limited cell quantity in culture flasks and the challenges of cell collection, we utilized 10-cm Petri dishes for cell harvesting, with each experimental group comprising five replicate samples. For complete details on the use and execution of this protocol, please refer to Zhang et al.1.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células Epiteliais
/
Rim
Limite:
Humans
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China