Cell Surface Parameters for Accessing Neutrophil Activation Level with Atomic Force Microscopy.
Cells
; 13(4)2024 Feb 07.
Article
em En
| MEDLINE
| ID: mdl-38391919
ABSTRACT
In this study, we examine the topography and adhesion images of the cell surface of neutrophils during the activation process. Our analysis of cell surface parameters indicates that the most significant changes in neutrophils occur within the first 30 min of activation, suggesting that reactive oxygen species may require approximately this amount of time to activate the cells. Interestingly, we observed surface granular structure as early as 10 min after neutrophil activation when examining atomic force microscopy images. This finding aligns with the reorganization observed within the cells under confocal laser scanning microscopy. By analyzing the cell surface images of adhesion, we identified three spatial surface parameters that correlate with the activation time. This finding enables us to estimate the degree of activation by using atomic force microscopy maps of the cell surface.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Ativação de Neutrófilo
Idioma:
En
Revista:
Cells
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Federação Russa