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Piperine, a phytochemical prevents the biofilm city of methicillin-resistant Staphylococcus aureus: A biochemical approach to understand the underlying mechanism.
Das, Sharmistha; Malik, Moumita; Dastidar, Debabrata Ghosh; Roy, Ritwik; Paul, Payel; Sarkar, Sarita; Chakraborty, Poulomi; Maity, Alakesh; Dasgupta, Monikankana; Gupta, Anirban Das; Chatterjee, Sudipta; Sarker, Ranojit Kumar; Maiti, Debasish; Tribedi, Prosun.
Afiliação
  • Das S; Microbial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India. Electronic address: sharmisthad986@gmail.com.
  • Malik M; Microbial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India. Electronic address: moumitamalik2019@gmail.com.
  • Dastidar DG; Guru Nanak Institute of Pharmaceutical Science and Technology, 157/F Nilgunj Road, Panihati, Kolkata, West Bengal, 700114, India. Electronic address: debabrata.ghoshdastidar@gnipst.ac.in.
  • Roy R; Microbial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India. Electronic address: ritwikroy096@gmail.com.
  • Paul P; Microbial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India. Electronic address: payelpaul17@gmail.com.
  • Sarkar S; Microbial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India. Electronic address: sarita_sarkar@yahoo.in.
  • Chakraborty P; Microbial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India. Electronic address: polochak12@gmail.com.
  • Maity A; Microbial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India. Electronic address: maityalakesh.2211.am@gmail.com.
  • Dasgupta M; Microbial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India. Electronic address: monikankana.dasgupta@gmail.com.
  • Gupta AD; Microbial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India. Electronic address: dasgupta.anirban42@gmail.com.
  • Chatterjee S; Microbial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India. Electronic address: kool.sudipta@gmail.com.
  • Sarker RK; Microbial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India. Electronic address: ranojitsarker@gmail.com.
  • Maiti D; Department of Human Physiology, Tripura University, Suryamaninagar, Agartala, Tripura, 799022, India. Electronic address: debumaiti@gmail.com.
  • Tribedi P; Microbial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India. Electronic address: tribedi.prosun@gmail.com.
Microb Pathog ; 189: 106601, 2024 Apr.
Article em En | MEDLINE | ID: mdl-38423404
ABSTRACT
Methicillin-resistant Staphylococcus aureus (MRSA), a drug-resistant human pathogen causes several nosocomial as well as community-acquired infections involving biofilm machinery. Hence, it has gained a wide interest within the scientific community to impede biofilm-induced MRSA-associated health complications. The current study focuses on the utilization of a natural bioactive compound called piperine to control the biofilm development of MRSA. Quantitative assessments like crystal violet, total protein recovery, and fluorescein-di-acetate (FDA) hydrolysis assays, demonstrated that piperine (8 and 16 µg/mL) could effectively compromise the biofilm formation of MRSA. Light and scanning electron microscopic image analysis confirmed the same. Further investigation revealed that piperine could reduce extracellular polysaccharide production by down-regulating the expression of icaA gene. Besides, piperine could reduce the cell-surface hydrophobicity of MRSA, a crucial factor of biofilm formation. Moreover, the introduction of piperine could interfere with microbial motility indicating the interaction of piperine with the quorum-sensing components. A molecular dynamics study showed a stable binding between piperine and AgrA protein (regulator of quorum sensing) suggesting the possible meddling of piperine in quorum-sensing of MRSA. Additionally, the exposure to piperine led to the accumulation of intracellular reactive oxygen species (ROS) and potentially heightened cell membrane permeability in inhibiting microbial biofilm formation. Besides, piperine could reduce the secretion of diverse virulence factors from MRSA. Further exploration revealed that piperine interacted with extracellular DNA (e-DNA), causing disintegration by weakening the biofilm architecture. Conclusively, this study suggests that piperine could be a potential antibiofilm molecule against MRSA-associated biofilm infections.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Piperidinas / Infecções Estafilocócicas / Alcaloides / Benzodioxóis / Alcamidas Poli-Insaturadas / Staphylococcus aureus Resistente à Meticilina Limite: Humans Idioma: En Revista: Microb Pathog Assunto da revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Piperidinas / Infecções Estafilocócicas / Alcaloides / Benzodioxóis / Alcamidas Poli-Insaturadas / Staphylococcus aureus Resistente à Meticilina Limite: Humans Idioma: En Revista: Microb Pathog Assunto da revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article