Characterization of Ras Y4H mutants in Drosophila.
MicroPubl Biol
; 20242024.
Article
em En
| MEDLINE
| ID: mdl-38495589
ABSTRACT
Ras signaling plays a highly conserved role from flies to mammals in establishing proper development, and its dysregulation can lead to cancer. In Drosophila , we demonstrated that Ras Tyrosine 4 (Y4) was required for inhibitory ubiquitination by Rabex-5. In humans, rare histidine substitution mutations at Y4 are found in HRas in cerebellar glioblastomas (cGBMs). We report here that analogous Y4H mutations in Drosophila Ras make it less sensitive to Rabex-5-mediated ubiquitination in cells and show increased frequency of vein phenotypes per wing compared to wild-type Ras, which would be consistent with Ras gain-of-function and with their appearance in human cGBMs.
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MEDLINE
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En
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MicroPubl Biol
Ano de publicação:
2024
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Article