ß-Dicarbonyls Facilitate Engineered Microbial Bromoform Biosynthesis.
ACS Synth Biol
; 13(5): 1492-1497, 2024 05 17.
Article
em En
| MEDLINE
| ID: mdl-38525720
ABSTRACT
Ruminant livestock produce around 24% of global anthropogenic methane emissions. Methanogenesis in the animal rumen is significantly inhibited by bromoform, which is abundant in seaweeds of the genus Asparagopsis. This has prompted the development of livestock feed additives based on Asparagopsis to mitigate methane emissions, although this approach alone is unlikely to satisfy global demand. Here we engineer a non-native biosynthesis pathway to produce bromoform in vivo with yeast as an alternative biological source that may enable sustainable, scalable production of bromoform by fermentation. ß-dicarbonyl compounds with low pKa values were identified as essential substrates for bromoform production and enabled bromoform synthesis in engineered Saccharomyces cerevisiae expressing a vanadate-dependent haloperoxidase gene. In addition to providing a potential route to the sustainable biological production of bromoform at scale, this work advances the development of novel microbial biosynthetic pathways for halogenation.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Engenharia Metabólica
Limite:
Animals
Idioma:
En
Revista:
ACS Synth Biol
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Austrália