E. coli Nickel-Iron Hydrogenase 1 Catalyses Non-native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme Ene-reductases.
Angew Chem Weinheim Bergstr Ger
; 133(25): 13943-13947, 2021 Jun 14.
Article
em En
| MEDLINE
| ID: mdl-38529476
ABSTRACT
A new activity for the [NiFe] uptake hydrogenaseâ
1 of Escherichia coli (Hyd1) is presented. Direct reduction of biological flavin cofactors FMN and FAD is achieved using H2 as a simple, completely atom-economical reductant. The robust nature of Hyd1 is exploited for flavin reduction across a broad range of temperatures (25-70 °C) and extended reaction times. The utility of this system as a simple, easy to implement FMNH2 or FADH2 regenerating system is then demonstrated by supplying reduced flavin to Old Yellow Enzyme "ene-reductases" to support asymmetric alkene reductions with up to 100 % conversion. Hyd1 turnover frequencies up to 20.4â
min-1 and total turnover numbers up to 20 200 were recorded during flavin recycling.
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1
Base de dados:
MEDLINE
Idioma:
En
Revista:
Angew Chem Weinheim Bergstr Ger
Assunto da revista:
BIOFISICA
/
QUIMICA
Ano de publicação:
2021
Tipo de documento:
Article