Characterization of Solitalea canadensis α-mannosidase with specific activity towards α1,3-Mannosidic linkages.
Carbohydr Res
; 538: 109100, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38555657
ABSTRACT
A recombinant exo-α-mannosidase from Solitalea canadensis (Sc3Man) has been characterized to exhibit strict specificity for hydrolyzing α1,3-mannosidic linkages located at the non-reducing end of glycans containing α-mannose. Enzymatic characterization revealed that Sc3Man operates optimally at a pH of 5.0 and at a temperature of 37 °C. The enzymatic activity was notably enhanced twofold in the presence of Ca2+ ions, emphasizing its potential dependency on this metal ion, while Cu2+ and Zn2+ ions notably impaired enzyme function. Sc3Man was able to efficiently cleave the terminal α1,3 mannose residue from various high-mannose N-glycan structures and from the model glycoprotein RNase B. This work not only expands the categorical scope of bacterial α-mannosidases, but also offers new insight into the glycan metabolism of S. canadensis, highlighting the enzyme's utility for glycan analysis and potential biotechnological applications.
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Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Bacteroidetes
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Manose
Idioma:
En
Revista:
Carbohydr Res
Ano de publicação:
2024
Tipo de documento:
Article