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Identification and quantification of pigments in plant leaves using thin layer chromatography-Raman spectroscopy (TLC-Raman).
Payne, Taylor D; Dixon, Lily R; Schmidt, Fiona C; Blakeslee, Joshua J; Bennett, Alison E; Schultz, Zachary D.
Afiliação
  • Payne TD; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA. schultz.133@osu.edu.
  • Dixon LR; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA. schultz.133@osu.edu.
  • Schmidt FC; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA. schultz.133@osu.edu.
  • Blakeslee JJ; Department of Horticulture and Crop Sciences, The Ohio State University, Columbus, Ohio 43210, USA.
  • Bennett AE; Laboratory for the Analysis of Metabolites from Plants (LAMP) Metabolomics Facility, The Ohio State University, Columbus, Ohio, 43210, USA.
  • Schultz ZD; Department of Evolution, Ecology, and Organismal Biology, The Ohio State University, Columbus, Ohio 43210, USA.
Anal Methods ; 16(16): 2449-2455, 2024 04 25.
Article em En | MEDLINE | ID: mdl-38563199
ABSTRACT
Carotenoids are yellow, orange, and red pigments commonly found in plants. In leaves, these molecules are essential for photosynthesis, but they also play a major role in plant growth and development. Efficiently monitoring concentrations of specific carotenoids in plant tissues could help to explain plant responses to environmental stressors, infection and disease, fertilization, and other conditions. Previously, Raman methods have been used to demonstrate a correlation between plant fitness and the carotenoid content of leaves. Due to solvatochromatic effects and structural similarities within the carotenoid family, current Raman spectroscopy techniques struggle to assign signals to specific carotenoids with certainty, complicating the determination of amounts of individual carotenoids present in a sample. In this work, we use thin layer chromatography-Raman spectroscopy, or TLC-Raman, to identify and quantify carotenoids extracted from tomato leaves. These quick and accurate methods could be applied to study the relationship between pigment content and a number of factors affecting plant health.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Análise Espectral Raman / Carotenoides / Solanum lycopersicum / Folhas de Planta Idioma: En Revista: Anal Methods Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Análise Espectral Raman / Carotenoides / Solanum lycopersicum / Folhas de Planta Idioma: En Revista: Anal Methods Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos