Profiling of Groundnut bud necrosis orthotospovirus-responsive microRNA and their targets in tomato based on deep sequencing.
J Virol Methods
; 327: 114924, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38574773
ABSTRACT
Tomato, an extensively cultivated vegetable crop produces miRNAs in response to infection with Groundnut bud necrosis orthotospovirus, a viral pathogen causing significant economic losses. High-throughput miRNA sequencing was performed on tomato leaves inoculated with GBNV and mock-inoculated leaves as controls. Analysis revealed 73 known miRNAs belonging to 24 miRNA families, with variable expression levels. Interestingly, 39 miRNAs were upregulated, and 34 were downregulated in response to GBNV infection. Stem-loop quantitative reverse transcription PCR validated the differential expression of selected miRNAs. Additionally, 30 miRNA encoded proteins were identified to be involved in disease resistance and susceptibility. The miRNA-target interactions were found to play significant roles in cellular and metabolic activities, as well as modulating signaling pathways during the plant-virus interaction. The findings shed light on the intricate regulatory network of miRNAs in tomato response to viral infection and may contribute to developing strategies for improving crop protection against viral diseases.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
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Tospovirus
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Solanum lycopersicum
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Folhas de Planta
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MicroRNAs
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Sequenciamento de Nucleotídeos em Larga Escala
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
2024
Tipo de documento:
Article