Pre- and Postembedding Immunoelectron Microscopy to Analyze the Central Nervous System.
Methods Mol Biol
; 2794: 45-62, 2024.
Article
em En
| MEDLINE
| ID: mdl-38630219
ABSTRACT
Immunocytochemistry, a method of delineating the subcellular localization of target proteins, was developed from immunohistochemistry. In principle, proteins are labeled using an antigen-antibody reaction. In order to observe under an electron microscope, the reaction product must scatter the electron beam with sufficient contrast while it is necessary to have an amplifying label that can withstand the observation. We have some detailed tips on making electron microscope samples to achieve this objective, and we would be happy to help you.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Sistema Nervoso Central
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Japão