Validation of GCN5L1/BLOC1S1/BLOS1 antibodies using knockout cells and tissue.
Biochem J
; 481(10): 643-651, 2024 May 22.
Article
em En
| MEDLINE
| ID: mdl-38683688
ABSTRACT
GCN5L1, also known as BLOC1S1 and BLOS1, is a small intracellular protein involved in many key biological processes. Over the last decade, GCN5L1 has been implicated in the regulation of protein lysine acetylation, energy metabolism, endo-lysosomal function, and cellular immune pathways. An increasing number of published papers have used commercially-available reagents to interrogate GCN5L1 function. However, in many cases these reagents have not been rigorously validated, leading to potentially misleading results. In this report we tested several commercially-available antibodies for GCN5L1, and found that two-thirds of those available did not unambiguously detect the protein by western blot in cultured mouse cells or ex vivo liver tissue. These data suggest that previously published studies which used these unverified antibodies to measure GCN5L1 protein abundance, in the absence of other independent methods of corroboration, should be interpreted with appropriate caution.
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Base de dados:
MEDLINE
Assunto principal:
Anticorpos
Limite:
Animals
Idioma:
En
Revista:
Biochem J
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos