Repair of Sciatic Nerve Defect in Rats With Acellular Nerve Allograft Carrying Vascular Endothelial Cells.
Ann Plast Surg
; 92(5): 585-590, 2024 May 01.
Article
em En
| MEDLINE
| ID: mdl-38685498
ABSTRACT
BACKGROUND:
Acellular nerve allografts (ANAs) were developed to replace the autologous nerve grafts (ANGs) to fill the peripheral nerve defects. Poor vascularization relative to ANGs has been a limitation of application of ANAs.METHODS:
A total of 60 female Sprague-Dawley rats were assigned 3 groups. The rats in A group received ANGs, the rats in B group received ANAs, and the rats in C group were transplanted with ANA carrying endothelial cells (ANA + ECs). In the 1st, 2nd, 4th, and 12th postoperative weeks, 5 rats were selected from each group for evaluating sciatic function index (SFI), electrophysiology, maximum tetanic force recovery rate, tibialis anterior muscle weights recovery rate, and microvessel density. In the 12th postoperative week, the nerves were harvested and stained with toluidine blue and observed under an electron microscope to compare nerve fibers, myelin width, and G-ratio.RESULTS:
All the rats survived. In the first and second postoperative weeks, more microvessels were found in the ANA + EC group. In the 12th postoperative week, the nerve fibers were more numerous, and G-ratio was smaller in the C group compared with the B group. The compound muscle action potential and maximum tetanic force recovery rate in the tibialis anterior muscle in the C group were better than those in the B group in the 12th postoperative week. The A group showed better performances in electrophysiology, maximum tetanic force, muscle wet weight, and nerve regeneration.CONCLUSION:
ANA + ECs can promote early angiogenesis, promoting nerve regeneration and neurological function recovery.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nervo Isquiático
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Ratos Sprague-Dawley
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Células Endoteliais
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Aloenxertos
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Regeneração Nervosa
Limite:
Animals
Idioma:
En
Revista:
Ann Plast Surg
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China