Species-wide quantitative transcriptomes and proteomes reveal distinct genetic control of gene expression variation in yeast.
Proc Natl Acad Sci U S A
; 121(19): e2319211121, 2024 May 07.
Article
em En
| MEDLINE
| ID: mdl-38696467
ABSTRACT
Gene expression varies between individuals and corresponds to a key step linking genotypes to phenotypes. However, our knowledge regarding the species-wide genetic control of protein abundance, including its dependency on transcript levels, is very limited. Here, we have determined quantitative proteomes of a large population of 942 diverse natural Saccharomyces cerevisiae yeast isolates. We found that mRNA and protein abundances are weakly correlated at the population gene level. While the protein coexpression network recapitulates major biological functions, differential expression patterns reveal proteomic signatures related to specific populations. Comprehensive genetic association analyses highlight that genetic variants associated with variation in protein (pQTL) and transcript (eQTL) levels poorly overlap (3%). Our results demonstrate that transcriptome and proteome are governed by distinct genetic bases, likely explained by protein turnover. It also highlights the importance of integrating these different levels of gene expression to better understand the genotype-phenotype relationship.
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MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Regulação Fúngica da Expressão Gênica
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Proteoma
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Proteínas de Saccharomyces cerevisiae
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Locos de Características Quantitativas
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Transcriptoma
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
França