Your browser doesn't support javascript.
loading
Stress phenotyping analysis leveraging autofluorescence image sequences with machine learning.
Das Choudhury, Sruti; Guadagno, Carmela Rosaria; Bashyam, Srinidhi; Mazis, Anastasios; Ewers, Brent E; Samal, Ashok; Awada, Tala.
Afiliação
  • Das Choudhury S; School of Natural Resources, University of Nebraska-Lincoln, Lincoln, NE, United States.
  • Guadagno CR; School of Computing, University of Nebraska-Lincoln, Lincoln, NE, United States.
  • Bashyam S; Department of Botany, University of Wyoming, Laramie, WY, United States.
  • Mazis A; School of Computing, University of Nebraska-Lincoln, Lincoln, NE, United States.
  • Ewers BE; School of Natural Resources, University of Nebraska-Lincoln, Lincoln, NE, United States.
  • Samal A; Department of Botany, University of Wyoming, Laramie, WY, United States.
  • Awada T; School of Computing, University of Nebraska-Lincoln, Lincoln, NE, United States.
Front Plant Sci ; 15: 1353110, 2024.
Article em En | MEDLINE | ID: mdl-38708393
ABSTRACT

Background:

Autofluorescence-based imaging has the potential to non-destructively characterize the biochemical and physiological properties of plants regulated by genotypes using optical properties of the tissue. A comparative study of stress tolerant and stress susceptible genotypes of Brassica rapa with respect to newly introduced stress-based phenotypes using machine learning techniques will contribute to the significant advancement of autofluorescence-based plant phenotyping research.

Methods:

Autofluorescence spectral images have been used to design a stress detection classifier with two classes, stressed and non-stressed, using machine learning algorithms. The benchmark dataset consisted of time-series image sequences from three Brassica rapa genotypes (CC, R500, and VT), extreme in their morphological and physiological traits captured at the high-throughput plant phenotyping facility at the University of Nebraska-Lincoln, USA. We developed a set of machine learning-based classification models to detect the percentage of stressed tissue derived from plant images and identified the best classifier. From the analysis of the autofluorescence images, two novel stress-based image phenotypes were computed to determine the temporal variation in stressed tissue under progressive drought across different genotypes, i.e., the average percentage stress and the moving average percentage stress.

Results:

The study demonstrated that both the computed phenotypes consistently discriminated against stressed versus non-stressed tissue, with oilseed type (R500) being less prone to drought stress relative to the other two Brassica rapa genotypes (CC and VT).

Conclusion:

Autofluorescence signals from the 365/400 nm excitation/emission combination were able to segregate genotypic variation during a progressive drought treatment under a controlled greenhouse environment, allowing for the exploration of other meaningful phenotypes using autofluorescence image sequences with significance in the context of plant science.
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: Front Plant Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: Front Plant Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos