Your browser doesn't support javascript.
loading
Perivascular neurons instruct 3D vascular lattice formation via neurovascular contact.
Toma, Kenichi; Zhao, Mengya; Zhang, Shaobo; Wang, Fei; Graham, Hannah K; Zou, Jun; Modgil, Shweta; Shang, Wenhao H; Tsai, Nicole Y; Cai, Zhishun; Liu, Liping; Hong, Guiying; Kriegstein, Arnold R; Hu, Yang; Körbelin, Jakob; Zhang, Ruobing; Liao, Yaping Joyce; Kim, Tyson N; Ye, Xin; Duan, Xin.
Afiliação
  • Toma K; Department of Ophthalmology, University of California, San Francisco, San Francisco, CA, USA.
  • Zhao M; Department of Ophthalmology, University of California, San Francisco, San Francisco, CA, USA.
  • Zhang S; Department of Ophthalmology, University of California, San Francisco, San Francisco, CA, USA.
  • Wang F; Department of Ophthalmology, University of California, San Francisco, San Francisco, CA, USA.
  • Graham HK; Department of Ophthalmology, University of California, San Francisco, San Francisco, CA, USA.
  • Zou J; Department of Discovery Oncology, Genentech Inc., South San Francisco, CA, USA.
  • Modgil S; Department of Ophthalmology, Stanford University School of Medicine, Palo Alto, CA, USA.
  • Shang WH; Department of Ophthalmology, University of California, San Francisco, San Francisco, CA, USA.
  • Tsai NY; Department of Ophthalmology, University of California, San Francisco, San Francisco, CA, USA.
  • Cai Z; Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
  • Liu L; Department of Ophthalmology, Stanford University School of Medicine, Palo Alto, CA, USA.
  • Hong G; Department of Ophthalmology, University of California, San Francisco, San Francisco, CA, USA.
  • Kriegstein AR; Department of Neurology and The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA, USA.
  • Hu Y; Department of Ophthalmology, Stanford University School of Medicine, Palo Alto, CA, USA.
  • Körbelin J; ENDomics Lab, Department of Oncology, Hematology and Bone Marrow Transplantation, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
  • Zhang R; Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
  • Liao YJ; Department of Ophthalmology, Stanford University School of Medicine, Palo Alto, CA, USA.
  • Kim TN; Department of Ophthalmology, University of California, San Francisco, San Francisco, CA, USA.
  • Ye X; Department of Discovery Oncology, Genentech Inc., South San Francisco, CA, USA. Electronic address: ye.xin@gene.com.
  • Duan X; Department of Ophthalmology, University of California, San Francisco, San Francisco, CA, USA; Department of Physiology and Kavli Institute for Fundamental Neuroscience, University of California, San Francisco, San Francisco, CA, USA. Electronic address: xin.duan@ucsf.edu.
Cell ; 187(11): 2767-2784.e23, 2024 May 23.
Article em En | MEDLINE | ID: mdl-38733989
ABSTRACT
The vasculature of the central nervous system is a 3D lattice composed of laminar vascular beds interconnected by penetrating vessels. The mechanisms controlling 3D lattice network formation remain largely unknown. Combining viral labeling, genetic marking, and single-cell profiling in the mouse retina, we discovered a perivascular neuronal subset, annotated as Fam19a4/Nts-positive retinal ganglion cells (Fam19a4/Nts-RGCs), directly contacting the vasculature with perisomatic endfeet. Developmental ablation of Fam19a4/Nts-RGCs led to disoriented growth of penetrating vessels near the ganglion cell layer (GCL), leading to a disorganized 3D vascular lattice. We identified enriched PIEZO2 expression in Fam19a4/Nts-RGCs. Piezo2 loss from all retinal neurons or Fam19a4/Nts-RGCs abolished the direct neurovascular contacts and phenocopied the Fam19a4/Nts-RGC ablation deficits. The defective vascular structure led to reduced capillary perfusion and sensitized the retina to ischemic insults. Furthermore, we uncovered a Piezo2-dependent perivascular granule cell subset for cerebellar vascular patterning, indicating neuronal Piezo2-dependent 3D vascular patterning in the brain.
Assuntos
Palavras-chave
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Retina / Cerebelo / Neurônios Limite: Animals Idioma: En Revista: Cell Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Retina / Cerebelo / Neurônios Limite: Animals Idioma: En Revista: Cell Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos