Yanghanlia caeni gen. nov., sp. nov., a novel taxon within the family Alcaligenaceae isolated from sludge of a pesticide-manufacturing factory.
Int J Syst Evol Microbiol
; 74(5)2024 May.
Article
em En
| MEDLINE
| ID: mdl-38767617
ABSTRACT
A Gram-stain-negative bacterium, designated LG-2T, was isolated from sludge collected at a pesticide-manufacturing factory in Jiangsu Province, PR China. Cells of strain LG-2T were strictly aerobic, non-motile and spherical. Growth was observed at 15-42â°C (optimum, 30â°C), pH 6.0-9.0 (optimum, pH 7.0) and 0-3.0â% (w/v) NaCl (optimum, 1.0â%). LG-2T showed 95.5-96.9â% 16S rRNA sequence similarity to type strains in the genera Pusillimonas, Bordetella, Parapusillimonas, Candidimonas and Paracandidimonas of the family Alcaligenaceae. The phylogenomic tree indicated that strain LG-2T was clustered in the family Alcaligenaceae and formed a clade with Paracandidimonas soli IMT-305T, while the phylogenetic trees based on 16S rRNA gene sequences indicated that strain LG-2T formed a distinct clade within the family Alcaligenaceae. The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between LG-2T and its closely related type strains in the genera Pusillimonas, Bordetella, Parapusillimonas, Candidimonas and Paracandidimonas were 70.8-75.3, 18.9-23.7 and 59.6â%-69.3â%, respectively. The major cellular fatty acids were C16ââ0, C17ââ0 cyclo, summed feature 3 (C16ââ1 ω7c and/or C16ââ1 ω6c), summed feature 8 (C18ââ1 ω7c and/or C18ââ1 ω6c) and summed feature 2 (C12ââ0 aldehyde and/or unknown 10.928). The predominant menaquinone was Q-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, three aminolipids and nine unknown polar lipids. The genome size of strain LG-2T was 3.2 Mb and the DNA G+C content was 63.4 mol%. On the basis of the phenotypic, phylogenetic and genomic results from this study, strain LG-2T represents a novel species of a new genus in the family Alcaligenaceae, for which the name Yanghanlia caeni gen. nov., sp. nov. is proposed, with strain LG-2T (=KCTC 8084T= CCTCC AB 2023123T) as the type strain.
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Base de dados:
MEDLINE
Assunto principal:
Filogenia
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Esgotos
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Composição de Bases
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DNA Bacteriano
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RNA Ribossômico 16S
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Técnicas de Tipagem Bacteriana
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Análise de Sequência de DNA
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Alcaligenaceae
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Ácidos Graxos
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Hibridização de Ácido Nucleico
País/Região como assunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2024
Tipo de documento:
Article