Achieving temperature stability for storage of biological samples in an autodefrost freezer.

ABSTRACT

OBJECTIVE: To assess the temperature stability of an autodefrost freezer commonly used in veterinary practices, whether the use of a Styrofoam cooler within the freezer provides temperature stability, and the ease of use of a remote monitoring system for the notification of temperature elevations. ANIMALS None. METHODS: Temperature in the freezer and 2 Styrofoam coolers were assessed with remote monitoring thermometers every 15 minutes. Temperature values were monitored from October 11 to December 18, 2023 (for a 68-day period). Data analysis focused on temperatures for the freezer exceeding 0 °C and the elevations in temperatures within the coolers relative to the freezer. RESULTS: The freezer had an increase in temperature approximately every 16 hours. Over 68 days, the freezer had a temperature greater than 0 °C 27 times, representing 26 separate elevations. The Styrofoam coolers within the freezer never registered a temperature higher than -5 °C. Elevations in temperature within the freezer were larger in magnitude than temperature elevations within the coolers, which showed smaller-magnitude changes in temperature. CLINICAL RELEVANCE The temperature stability provided by the Styrofoam cooler would avoid potential freeze-thaw cycles of any stored biological samples. Additionally, the remote temperature monitoring system is easy to install and monitor, providing peace of mind to practice management.