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Acidithiobacillus acidisediminis sp. nov., an acidophilic sulphur-oxidizing chemolithotroph isolated from acid mine drainage sediment.
Li, Xiu-Tong; Liang, Zong-Lin; Huang, Ye; Jiang, Zhen; Yang, Zhen-Ni; Zhou, Nan; Liu, Ying; Liu, Shuang-Jiang; Jiang, Cheng-Ying.
Afiliação
  • Li XT; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
  • Liang ZL; University of Chinese Academy of Sciences, Beijing 100049, PR China.
  • Huang Y; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
  • Jiang Z; University of Chinese Academy of Sciences, Beijing 100049, PR China.
  • Yang ZN; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
  • Zhou N; University of Chinese Academy of Sciences, Beijing 100049, PR China.
  • Liu Y; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
  • Liu SJ; University of Chinese Academy of Sciences, Beijing 100049, PR China.
  • Jiang CY; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Article em En | MEDLINE | ID: mdl-38805027
ABSTRACT
Strain S30A2T, isolated from the acid mine drainage sediment of Mengzi Copper Mine, Yunnan, is proposed to represent a novel species of the sulphur-oxidizing genus Acidithiobacillus. Cells were Gram-stain-negative, non-endospore forming, highly motile with one or two monopolar flagella and rod-shaped. The strain was mesophilic, growing at 30-50 °C (optimum, 38 °C), acidophilic, growing at pH 2.0-4.5 (optimum, pH 2.5), and tolerant of 0-4 % (w/v; 684 mol l-1) NaCl. The 16S rRNA gene-based sequence analysis showed that strain S30A2T belongs to the genus Acidithiobacillus and shows the largest similarity of 96.6 % to the type strain Acidithiobacillus caldus KUT. The genomic DNA G+C content of strain S30A2T was 59.25 mol%. The average nucleotide identity ANIb and ANIm values between strain S30A2T and A. caldus KUT were 70.95 and 89.78 %, respectively and the digital DNA-DNA hybridization value was 24.9 %. Strain S30A2T was strictly aerobic and could utilize elementary sulphur and tetrathionate to support chemolithotrophic growth. The major cellular fatty acid of S30A2T was C19  1ω7c. The respiratory quinones were ubiquinone-8 and ubiquinone-7. Based upon its phylogenetic, genetic, phenotypic, physiologic and chemotaxonomic characteristics, strain S30A2T is considered to represent a novel species of the genus Acidithiobacillus, for which the name Acidithiobacillus acidisediminis sp. nov. is proposed. The type strain is S30A2T (=CGMCC 1.17059T=KCTC 72580T).
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Filogenia / Enxofre / Composição de Bases / DNA Bacteriano / RNA Ribossômico 16S / Técnicas de Tipagem Bacteriana / Análise de Sequência de DNA / Sedimentos Geológicos / Acidithiobacillus / Ácidos Graxos País/Região como assunto: Asia Idioma: En Revista: Int J Syst Evol Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Filogenia / Enxofre / Composição de Bases / DNA Bacteriano / RNA Ribossômico 16S / Técnicas de Tipagem Bacteriana / Análise de Sequência de DNA / Sedimentos Geológicos / Acidithiobacillus / Ácidos Graxos País/Região como assunto: Asia Idioma: En Revista: Int J Syst Evol Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article