Tailored electrochemical biosensor with poly-diallydimethylammonium chloride-functionalised multiwalled carbon nanotubes/gold nanoparticles/manganese dioxide, and haemoglobin for sensitive hydrogen peroxide detection.
Talanta
; 276: 126290, 2024 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-38805755
ABSTRACT
A very sensitive electrochemical biosensor, with haemoglobin (Hb) as its basis, has been created to quantify hydrogen peroxide (H2O2), an essential marker in environmental monitoring, food safety, and medical diagnosis. The sensor uses a simple, eco-friendly preparation method. Hb was immobilised on manganese dioxide nanostructure/gold nanoparticles/poly-diallydimethylammonium chloride-functionalised multiwalled carbon nanotubes (PDDA-MWCNT/AuNP/MnO2), characterised using various techniques amperometry, voltammetry, X-ray diffraction (XRD), and transmission electron microscopy (TEM). Nafion was used as a binder membrane to preserve the biological and electrochemical properties of the protein on the modified electrode. In comparison to earlier research, the novel biosensor had a lower detection limit (1.83 µM) and a limit of quantification (6.11 µM) (S/N = 3) for H2O2. It also exhibited notable reproducibility, long-term stability, and repeatability. It was effectively used to measure the amount of H2O2 in cow milk and orange juice, yielding recoveries in the order of 98.90-99.53 % with RSDs less than 5.0 %, which makes it a promising biosensor for food control.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Óxidos
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Hemoglobinas
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Técnicas Biossensoriais
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Compostos de Manganês
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Nanotubos de Carbono
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Leite
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Nanopartículas Metálicas
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Técnicas Eletroquímicas
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Ouro
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Compostos de Amônio Quaternário
Limite:
Animals
Idioma:
En
Revista:
Talanta
Ano de publicação:
2024
Tipo de documento:
Article