Design and fabrication of 3D-printed in situ crystallization plates for probing microcrystals in an external electric field.
J Appl Crystallogr
; 57(Pt 3): 842-847, 2024 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-38846773
ABSTRACT
X-ray crystallography is an established tool to probe the structure of macromolecules with atomic resolution. Compared with alternative techniques such as single-particle cryo-electron microscopy and micro-electron diffraction, X-ray crystallography is uniquely suited to room-temperature studies and for obtaining a detailed picture of macromolecules subjected to an external electric field (EEF). The impact of an EEF on proteins has been extensively explored through single-crystal X-ray crystallography, which works well with larger high-quality protein crystals. This article introduces a novel design for a 3D-printed in situ crystallization plate that serves a dual purpose:
fostering crystal growth and allowing the concurrent examination of the effects of an EEF on crystals of varying sizes. The plate's compatibility with established X-ray crystallography techniques is evaluated.
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Base de dados:
MEDLINE
Idioma:
En
Revista:
J Appl Crystallogr
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
República Tcheca